ABSTRACT
Objective: To screen the anti-depressant active ingredients in Acori Tatarinowii Rhizoma by molecular docking technique. Methods: All the chemical constituents of Acori Tatarinowii Rhizoma were obtained by Traditional Chinese Medicine Pharmacology Database (TCMSP). Combined with oral bioavailability, 65 candidate compounds were obtained. The studied anti-depressant targets included monoamine oxidase A, dopamine transporter, serotonin transporter, and histamine H1 receptor. AutoDock Vina was used to explore the affinity and binding modes of the candidate compounds with the above targets. Results: There were 18 compounds from Acori Tatarinowii Rhizoma that could bind to the above four targets. Among them, four compounds could bind to four targets, three compounds could bind to two targets, and the other 11 compounds could interact with one target. Conclusion: Molecular docking technology can be used to investigate the molecular mechanism of Acori Tatarinowii Rhizoma and determine the active ingredients, which provides an alternative method for the discovery of novel anti-depressants and lead compounds from Chinese herbal medicines.
ABSTRACT
Serratia marcescens jn01 was employed as the host for the isolation of phages from environmental sewage. One strain of phage named SmPjn was purified by picking transparent plaque with 2mm diameter and clear edge on the double-layer agar repeatedly. Electron micrographs indicated that the phage head was icosahedral with head size and tail length of (58 +/- 2.16) x (55 +/- 0.47) nm and (7 +/- 1.25) nm, respectively. On the basis of the morphology, this phage belongs to the family Podoviridae. Host-range determination revealed that the phage was capable of infecting the other two isolates of S. marcescens, P25 and CMCC41002. The optimal multiplicity of infection was 1. A one-step growth curve of SmPjn indicated that the latent period and burst size were estimated at 50 min and 1,125 pfu/cell, respectively . Genomic DNA of SmPjn was above 27kb in size and could be digested by Hind Ill and EcoR I into 11 and 9 visible fragments after electrophoresis, respectively. A novel Podoviridae-phage infecting S. marcescens was firstly reported in China.
Subject(s)
China , DNA, Viral , Genetics , Metabolism , Host Specificity , Podoviridae , Genetics , Restriction Mapping , Serratia marcescens , PhysiologyABSTRACT
Objective To study the ultra-high cycle fatigue behaviors of the Ti-6Al-4V alloy implant after subjection to simulated body fluid (SBF) environment exceeding 107 cycles, and to predict its service life. Method The Ti-6Al-4V alloy specimens were subjected to SBF in two groups for two days and six days respectively; using the ultrasonic fatigue testing technique, the ultra high cycle fatigue properties of Ti-6Al-4V alloy after subjection to SBF in body temperature were studied and the initiation mechanisms of fatigue cracks were investigated and analyzed with scanning electron microscopy (SEM) and energy dispersive atomic X-ray (EDA X ray). Then, a comparison with corresponding behaviors of the normal Ti 6Al 4V alloy was made. Results The S~N curve of the Ti-6Al-4V alloy after subjection had the similar tendency as that of the normal one, showing that they descended continuously during 104 and 108 cycles and the descending tendency was lower in the region of 107～108 cycles; the life of the specimens after subjection dropped before the fatigue life of 107 cycles and was smaller after the fatigue life of 107 cycles than that of the normal ones; the life of the specimens subjected for six days had little difference with that subjected for two days; fracture could still occur beyond 107 cycles, showing that there was no fatigue limit as the traditional fatigue conception described. Fatigue cracks mainly initiated from the surface of specimen before the fatigue life of 107 cycles and some specimens had multiple crack initiation sites; fatigue failure initiated from internal inclusion where mostly the element Al aggregated after the fatigue life of 108 cycles. Conclusions The ultra high cycle fatigue properties of the Ti-6Al-4V alloy decreas a little with SBF subjection; the initiation site of the fatigue crack changes from the surface to the internal defect with the increase of the number of cycle.